Fn-Dps, a novel virulence factor of Fusobacterium nucleatum, disrupts erythrocytes and promotes metastasis in colorectal cancer.

Fn-Dps, a novel virulence factor of Fusobacterium nucleatum, disrupts erythrocytes and promotes metastasis in colorectal cancer.

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Fusobacterium nucleatum (Fn) is a critical colorectal cancer (CRC)-associated bacterium.DNA hunger/stationary phase protective proteins (Dps) are bacterial ferritins that chainsaw file protect DNA from oxidative stress.However, little is known about the regulatory roles of Fn-Dps towards host cellular functions.Here, we identified Fn-Dps from the culture supernatant of Fn by mass spectrometry, and prepared the recombinant of Fn-Dps protein.

We show a novel virulence protein of Fn, Fn-Dps, which lyses and disrupts erythrocytes by the competition for iron acquisition.Also, Fn-Dps facilitates intracellular survival of Fn in macrophages by upregulating the expression of the chemokine CCL2/CCL7.In addition, Fn-Dps can elicit a strong humoral immune response, and mucosal immunization with Fn-Dps conferred protection against Fn in the intestinal tract.Moreover, a high level of anti-Fn-Dps antibody was prevalent in populations, and elevated anti-Fn-Dps antibody levels were observed in CRC patients.

Furthermore, Fn-Dps promotes the migration of CRC cells via the CCL2/CCL7-induced socialstudiesscholar.com epithelial-mesenchymal transition (EMT) and promotes CRC metastasis in vivo.